The myelodysplastic syndromes (MDS) are a diverse set of cancers in the bone marrow.
The bone marrow consists of several cell types - amongst these hematopoietic stem cells and progenitor cells.
Gene expression analysis of specific cell fractions derived from the bone marrow could reveal potential targets of therapy and immune evasive mechanisms.
From: https://www.sigmaaldrich.com/
Materials and methods: Data Retrieval and Cleaning
Flowchart of data aquisition and inital tidying
Methods: Gene Mapping, Cleanup and DESeq2 Analysis
Flowchart of data augmentation
Sample compositions
Distribution of counts
Histogram normalized
Histogram non-normalized
Clustering using PCA on all cells and on HSC
Clustering analysis using K-means (k = 4)
Differential gene expression: All samples and HSC
Differential gene expression across the different cell populations - MDS vs. HD