Group 4 Project Presentation: Gene Expression in Bone Marrow CD34+ Hematopoietic Stem and Progenitor Cells
2024-12-03
Introduction
- The myelodysplastic syndromes (MDS) are a diverse set of cancers in the bone marrow.
- The bone marrow consists of several cell types - amongst these hematopoietic stem cells and progenitor cells.
- Gene expression analysis of specific cell fractions derived from the bone marrow could reveal potential targets of therapy and immune evasive mechanisms.
Materials and methods: Data Retrieval and Cleaning
Flowchart of data aquisition and inital tidying
Methods: Gene Mapping, Cleanup and DESeq2 Analysis
Flowchart of data augmentation
Sample compositions
Distribution of counts
Clustering using PCA on all cells and on HSC
Clustering analysis using K-means (k = 4)
Differential gene expression: All samples and HSC
Differential gene expression across the different cell populations - MDS vs. HD
